Journal: Frontiers in Genetics
Article Title: Circular RNA Circ_0013958 Functions as a Tumor Promoter in Ovarian Cancer by Regulating miR-637/PLXNB2 Axis
doi: 10.3389/fgene.2021.644451
Figure Lengend Snippet: Circ_0013958 was upregulated, while miR-637 was downregulated in OC tissues and cells. (A) QRT-PCR assay for the expression of circ_0013958 in OC tissues and adjacent normal tissues ( n = 30). (B) QRT-PCR analysis for the expression of circ_0013958 in OC cell line (SKOV3 and CAOV3) and human ovarian surface epithelial cells (HOSE). (C) ROC curve analysis of the prognostic sensitivity and specificity of circ_0013958 in OC patients in accordance with the 5-year survival of OC patients. (D) QRT-PCR assay for the expression of miR-637 in OC tissues and matched normal tissues ( n = 30). (E) QRT-PCR assay for the expression of miR-637 in HOSE, SKOV3, and CAOV3 cells. (F) Pearson's correlation analysis for the correlation between the expression levels of circ_0013958 and miR-637 in OC tissues ( n = 30). * P < 0.05; ** P < 0.01.
Article Snippet: Normal human ovarian surface epithelial cell line (HOSE; #7310, ScienCell Research Laboratories, Carlsbad, CA, USA) and OC cell lines SKOV3 (ATCC® HTB-77, American Type Culture Collection, Manassas, VA, USA) and CAOV3 (ATCC® HTB-75) were maintained in a Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Grand Island, NY, USA), added with 10% fetal bovine serum (FBS; HyClone, Logan, UT, USA) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37°C in the presence of 5% CO 2 .
Techniques: Quantitative RT-PCR, Expressing